Fig. 6.

POMC^N1-ICD downregulates FoxL2 and α-SMA expression during eyelid morphogenesis at E15.5. Immunofluorescent staining with anti-FoxL2 (red, A,B) or anti-skeletal muscle myosin (red, C,D) and anti-α-SMA (green, C,D). Note that FoxL2-positive signals (red) are downregulated in the POMC^N1-ICD (B) compared with POMC^wt (A). Arrows in C indicate α-SMA-positive smooth muscle sheet. Arrowhead in D indicates that POMC^N1-ICD failed to form α-SMA-positive muscle sheet. Other green signals are smooth muscle of the blood vessels. DAPI nuclear counterstain is shown in blue. The skeletal muscle expression pattern changed very little. Co, cornea; Le, Lens; LL, lower lid; Re, retina; UL, upper lid.