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Downregulation of α-SMA expression and eyelid levator smooth muscle malformation in Dox-treated KR/TC/R26fN1-ICD mice at different stages. (A–I) Immunofluorescent staining with anti-skeletal muscle myosin (red), α-SMA (green) and counterstaining with DAPI (blue) at P1 (A,D,G), P15 (B,E,H) and P60 (C,F,I). Arrows indicate α-SMA-positive eyelid smooth muscle sheet. Other green signals are smooth muscle of the blood vessels. Co, cornea; Le, Lens; LL, lower lid; Re, retina; UL, upper lid.
