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. 2011 Aug 1;124(15):2561–2572. doi: 10.1242/jcs.085001

Fig. 8.

Fig. 8.

Binding of RBP-Jκ and Hes-1 to the mFoxL2 promoter. (A) Predicted RBP-Jκ binding site and Hes-1 binding sites within mFoxL2 5′-flanking regulatory region (~3.1 kb). Arrows are DNA primers used in ChIP assay for RBP-Jκ binding site and Hes-1 binding sites. (B–D) ChIP analysis of RBP-Jκ binding to mouse FoxL2 promoter region. The binding region of RBP-Jκ to Hes-1 promoter served as ChIP positive control (C) and its non-binding region as negative control (D). Notice that both anti-Myc tag and anti-RBP-Jκ antibodies pull down RBP-Jκ binding region of the mFoxL2 promoter, indicating the binding of the N1-ICD–RBP-Jκ complex to the RBP-Jκ site of the mFoxL2 promoter. (E) ChIP analysis of Hes-1 binding to mouse FoxL2 promoter region. N-box sites 1, 2 and 4 show evident Hes-1 binding, but site 3 does not bind to the mouse FoxL2 promoter region.