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. 2011 Aug 1;124(15):2622–2630. doi: 10.1242/jcs.084798

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 5. — DILA–FLAG resides at the base of the cilium. (A) When expressed in larval Ch neurons, DILA–FLAG is localised at the basal body (bb) region, as indicated by anti-HRP antibody staining. The DILA–FLAG staining in the distal cilia (*) varies with the expression level and is presumed to be an artifact of protein overexpression. (B–B″) Sas-4 (magenta) reveals the distal and proximal basal bodies (db, pb) at the base of each cilium. DILA–FLAG staining overlaps with Sas-4 staining and is especially prominent in the cilium beyond the distal basal body, predicted to be the putative transition zone (tz). (C) When expressed at a low level, DILA–FLAG is confined to the putative transition zone. (D) Schematic of the cilium base showing the distribution of proteins.