Fig. 5.

Growth dynamics of microtubules in ROCK-inhibited cells is altered. (A) Untreated, DMSO, Y27632, and Y27362 + nocodazole-treated CEFs expressing GFP–EB1 (supplementary material Movies 5–7). Higher magnifications of EB1 comets present at the leading lamellae (white boxes) are shown below. Scale bar: 16 μm. (B) Comet speed of untreated CEFs and CEFs treated with DMSO and Y27632 (left); comet length (middle) and comet duration (right) of untreated CEFs and CEFs treated with DMSO, Y27632 and Y27632 + nocodazole. 30 individual comets per frame (from eight different time-points) of ten individual cells were used in each case; **P<0.01.