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. 2011 Jul 18;6(7):e21889. doi: 10.1371/journal.pone.0021889

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A- Immuno-EM of HeLa cells expressing GFP-tagged H. sapiens ATGL-S47A were treated with 200 µM oleic acid for 15 hours and labeled with gold-conjugated antibodies against GFP. The majority of gold particles (examples indicated by arrows) are found on the surface of LDs. LD, lipid droplet. B- Co-immunoprecipitations from Cos7 cells expressing HA-tagged H. sapiens ATGL-S47A or ATGL(1–366)-S47A and Venus-tagged GBF1 fragments were carried out as in Figures 1 and 4; Western blot of immunoprecipitates probed with anti-HA antibodies (upper panel); Cos7 cell extracts blotted with anti-GFP antibodies (lower panel).