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. 2011 Jul 18;6(7):e21889. doi: 10.1371/journal.pone.0021889

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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HeLa cells transfected with Venus-tagged HDS1 and/or HDS2 domains of GBF1 as indicated were treated with 400 µM oleic acid for 3 hours, then immunostained with antibodies against TIP47 (middle panels). Venus fluorescence is shown on the left; the merge image on the right. Bar: 10 µm. A- Cells expressing the region of GBF1 from the beginning of HDS1 to the end of HDS2, N-terminally tagged with Venus. B- Cells expressing the Venus-tagged HDS1 domain. C- Cells expressing Venus-HDS2. Bar, 10 µm.