Table 1. Yeast two-hybrid interactions between regions of GBF1 and ATGL.

	ATGL
GBF1	Full-length (M1-L504)	Patatin (M1-L178)	M1-M366	M1-L252	M366-L504	L300-L504	L300-T370	pGBKT7
DCB (V2-E202)	0	0	0	0	+	+	++	0
HUS* (P203–P363)	0	0	0	0	0	0	0	0
HUS-D544A* (P203–P363)	0	0	0	0	0	0	0	0
N (M1-N698)	0	0	0	0	0	0	0	0
N-D544A (M1-N698)	0	0	0	0	0	0	0	0
Sec7 (F710-V894)	0	0	0	0	+++	+	++	0
C (N896-S1856)	0	0	0	0	0	0	0	0
pGADT7	0	0	0	0	0	0	0	0

The indicated GBF1 fragments (first column) are in vector pGADT7 (amino acids at the beginning and end of each fragment are given), and the indicated portions of ATGL (first row) are in pGBKT7. Level of growth on –HIS-LEU-TRP and –ADE–HIS-LEU-TRP selective plates is indicated by plus signs (+); “0” indicates no growth on selective plates. *Note that these constructs differ from the HUS-GBF1 domain shown in Figure 2 in that the region between the HUS and Sec7 domains is not present.