Table 2. Summary of major interactions between GBF1 and ATGL.

Interaction	Methods used	Comments
GBF1 Full-lengthATGL Full-length	Co-IP:- Endogenous proteins- Tagged proteins expressed from plasmids	Interaction between full-length proteins is stronger when GBF1 HUS-DCB interaction is disrupted (by D544A mutation in HUS domain or deletion of DCB domain).
GBF1 DCB domainATGL C-terminus (aa300–504)	- Yeast 2-hybrid- Co-IP tagged fragments- Purified proteins	The GBF1 DCB domain interacts best with the hydrophobic domain (aa300–370) of the ATGL C-terminus.
GBF1 Sec7 domainATGL C-terminus (aa300–504)	- Yeast 2-hybrid- Co-IP tagged fragments- Purified proteins	Direct interaction with an apparent Kd of approximately 2.5 µM.
GBF1 HDS1 domainATGL patatin domain (N-term)	- Co-IP tagged fragments- Purified proteins	In co-IP experiments, GBF1 HDS2 also interacts with ATGL patatin domain.