Figure 6. Site-directed, randomizing mutagenesis of EFR sites that are divergent in EFR paralogs reveals many partial-impact LRR sites.

Seedling growth inhibition assays for EFR function were performed as in Figure 5 except that seedlings were treated with 1 uM elf18. A minimum of 65 T1 seedlings were tested for each allele library. Plants were classified as elf18-sensitive if their response was in the range of 95% of positive controls transformed with wild-type EFR, as elf18-insensitive if response was in the range of 95% of negative controls transformed with empty vector, or as partially sensitive if they fell within both ranges. WT: wild-type; EV: empty vector; allele number codes reflect the repeat number and the β-strand/β-turn position of the mutagenized amino acid (for example, 2.3 is the third x position of LxxLxLxxN in the second repeat), and parentheses enclose the amino acid number and letter.