Figure 1. Metabolomic profiling of prostate cell lines.

Illustration of the various steps involved in metabolomic profiling of prostate cell lines. The major steps involved metabolite extraction and separation, mass spectrometry-based detection, spectral analysis, data normalization, delineation of class-specific metabolites and altered pathways and their functional characterization. The variation in sample extraction, separation and mass spectrometry were controlled using spiked standards and assessed using various quality control parameters (Figure S1).