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. 2011 Jul 18;6(7):e20659. doi: 10.1371/journal.pone.0020659

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Rochiera et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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P19 cells were fixed with 4% PFA and permeabilized followed by application of primary antibodies for NRAGE and XIAP and widefield imaging. Secondary Alexa 488 and 546 antibodies were used to identify NRAGE (A) and XIAP (B) respectively. (C) Merged imaging shows NRAGE and XIAP mainly occupy the cytoplasm with smaller concentrations in the nucleus. (D) Phase contrast image.