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. 2011 Jul 18;6(7):e20659. doi: 10.1371/journal.pone.0020659

Figure 5. NRAGE repeat domain deletion mutations and XIAP interaction by FRET.

Figure 5

(A) Schematic of 4 designs of the repeat domain in NRAGE denoted by their primers as F10R3, F11R3, F12R3, and F13R3 and their fusion scheme with a EYFP fluorescent tag and (B) protein expression in P19 total cell lyses. (C) Peak energy transfer efficiency averages show progressively increasing FRET signals between ECFP-XIAP and NRAGE repeat domain deletions-EYFP when compared to controls (*P<0.05; **P<0.005; n is the number of images each containing 1 to 3 cells). C-X, ECFP-XIAP; Fx-Y, NRAGE FxR3-EYFP.