Table 4. Primer used to verify microarray experiments in liver tissue by qRT-PCR.

Gene name	Probe set ID	Sequence 5′ – 3′	Accession no.	T(C)	Size (bp)
DHCR7	Ssc.5455.1.S1_at	For GCATGACACTGACTTCTTCTC Rev CCCACCTCCACTTTATTC	BE232966	60	136
GADD45B	Ssc.14764.1.A1_at	For GGACTTAGACTTTGGGACTTG Rev GTAAGCCTCCCATCTCTCTT	BF708594	60	140
HMGCR	Ssc.16088.1.S1_at	For GTGCTGGTCTGTTTTGATTT Rev TGCAGTGATTTGTTTTCTTG	BP436947	60	159
NDUFA 4	Ssc.7315.1.S1_at	For TCCTGCTTAGTCCCCGACCTT Rev ACAGTGCTGCTCCAGTACCTCC	CF793329	60	164
NDUFS 7	Ssc.1681.1.S1_at	For CGGCTACTACCACTACTCCT Rev ATCCGCAGTCTCTTCTCC	CK455535	60	156
NDUFS 8	Ssc.2312.1.S1_at	For AGTTTGTGAACATGCGTGAG Rev CTCAAATGGGTAGTTGATGG	BI181006	60	152
PPARGC1A	Ssc.16864.1.S1_at	For GTAAATCTGCGGGATGATGG Rev TGGTGGAAGCAGGATCAAAG	AB106108	60	208
PRKAA 1	Ssc.8107.1.A1_at	For TTGTTAATTTCATAAACTTTGCTTC Rev GTGCAGCCTTGACATACTC	BF712533	60	193
PRKAA 2	Ssc.16257.1.S1_at	For TCTGTAATTCTGTTTTGCCTACG Rev AGCAAGAAGGTGATGCCAAG	NM214266	60	168
RPL 10*	Ssc.9130.1.A1_at	For CTGTGTTCGTCTTTTCTTCC Rev TCATCCACTTTTGCCTTCT	BI181297	60	199

DHCR7 - 7-dehydrocholesterol reductase; GADD45B - growth arrest and DNA-damage-inducible, beta; HMGCR - 3-hydroxy-3-methylglutaryl-coenzyme A reductase; NDUFA4 - NADH-ubiquinone oxidoreductase MLRQ subunit; NDUFS7 - NADH-ubiquinone oxidoreductase 20 kDa subunit; NDUFS8 - NADH-ubiquinone oxidoreductase 23 kDa subunit; PPARGC1A - Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PRKAA1 – 5′-AMP-activated protein kinase, catalytic alpha-1 chain; PRKAA2 – 5′-AMP-activated protein kinase, catalytic alpha-2 chain; RPL10 - Ribosomal protein 10.

*House keeping gene.