Figure 7. Cytokine responses to in vitro re-stimulation of tracheobronchial lymph node (TBLN) cells from A. baumannii-treated mice.

Groups of OVA-sensitized C57BL/6 mice were i.n. treated with either A. baumannii or PBS 7 days before i.n. OVA challenge. Mice were killed 5 days after the challenge and their TBLNs were collected and used for in vitro culture to determine cytokine production in response to OVA stimulation. Single cell suspensions (4×10⁶ cells/mL) were re-stimulated in vitro for 48 h with either OVA (1 mg/mL) or culture medium only. The cytokine levels in the supernatants were determined by Luminex. Data are presented as mean concentration (pg/ml) ± SD (n = 5), and are representative of two independent experiments.