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. 2011 Jul 18;6(7):e22004. doi: 10.1371/journal.pone.0022004

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Qiu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BAL from A. baumannii-treated and PBS-treated, allergic mice were collected at 5 days after i.n. OVA challenge and the percentage of regulatory T cells was calculated by flow cytometry as determined by CD4⁺CD25⁺Foxp3⁺ staining. A representative dot plot from an A. baumannii-treated (left panel) and a sham-treated (right panel) mouse illustrates the percentage of CD4⁺Foxp3⁺ Treg cells gated on total lymphocytes in the BAL fluid.