Figure 2. Inducible genetic targeting of Cdc42 inhibited filopodia and lamellipodia formation on immobilized collagen-related peptide.

Washed platelets from Cdc42^+/+ (A) and Cdc42^−/− (B) mice were layered over cover slips coated with CRP (0.5 µg/ml) for 20 minutes. The cover slips were washed and adherent platelets were processed for DIC (top panel) and immuno-fluorescence (bottom panel) microscopy as detailed in the methods section. Platelets from Cdc42^−/− mice failed to form filopodia or lamellipodia on CRP.