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. 2011 Jul 18;6(7):e22148. doi: 10.1371/journal.pone.0022148

Figure 3. Induction of SPI1 mRNA in K562 cells overexpressing V617F-type JAK2.

Figure 3

A. Western blots showing the amounts of JAK2 protein inK562 and HEL cells infected with retrovirus vector encoding either wild-type (JAK2 WT) or V617F-type JAK2 (JAK2 V617F) or a mock vector (vector) and maintained in the presence of puromycin. The intensities of bands were calibrated with the band intensities of elongation factor 2 (EF-2) protein. Fold over-expression is shown below as the value for the mock infectant as 1. The results of two independent infections are shown under experiments (exp.) 1 and 2. Alexa 680-labeled secondary antibodies were used. B. SPI1 mRNA levels in K562 cells prepared by the retroviral infection shown in A, along with those in non-infected K562 and HEL cells. The results were calibrated with 18S ribosomal RNA amount and represented with an arbitrary unit.