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. 2011 Jul 18;6(7):e22148. doi: 10.1371/journal.pone.0022148

Figure 4. Reduction of SOCS3 and SPI1 mRNA in HEL cells transfected with JAK2 siRNAs.

Figure 4

A. Western blots of JAK2 protein in HEL and K562 cells treated with siRNAs against JAK2 are shown. EF-2 was detected as a loading control. Cell lysates were prepared 24 h after siRNA transfection. Proteins derived from 1×105 cells were loaded onto each lane. Alexa 680-labeled secondary antibodies were used. Three types of siRNA against JAK2 (siRNA1–3) and a negative control siRNA (NC1) are described in the Materials and Methods section. Fold change represents a ratio of band intensity of JAK2 and that of EF-2. B. SOCS3 mRNA amount determined by qPCR. RNA was prepared 48 h after siRNA transfection. The values are expressed with an arbitrary unit as the mean of NC1 and NC2-treated HEL cells as 1. Error bars represent standard errors for triplicate measurements. C. SPI1 mRNA amount shown as in B.