Skip to main content
. 2011 Jul 18;6(7):e22014. doi: 10.1371/journal.pone.0022014

Figure 2. Mass determination and heme cleavage of cytochrome c-554 from Methylosinus trichosporium OB3b.

Figure 2

A. Mass spectrum of the native cytochrome c-554 from Methylosinus trichosporium OB3b. B. Mass spectrum of cytochrome c-554 after treatment with 2-NPS. The number of peaks is due to protein adducts with different number of added 2-NPS molecules. C. The reaction of 2-NPS with the covalent heme attachment, resulting in a disulphide link between 2-NPS and the protein and loss of the heme group. D. 2-NPS reacts with tryptophan, creating a chromophore with an absorption maximum at 365 nm [30].