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. 2011 Jul 18;6(7):e22233. doi: 10.1371/journal.pone.0022233

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Ng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Percentage of SLC19A3 promoter methylation between tumor tissues and their paired adjacent non-tumor breast tissues from the 15 breast cancer patients by MS-qPCR. Percentage of methylation in tissue samples was calculated by the following equation: % meth = 100/[1+2^{ΔCt(meth-unmeth)}]%. ΔCt_{(meth-unmeth)} was calculated by subtracting the Ct values of methylated SLC19A3 signal from the Ct values of umnethylated SLC19A3 signal. Statistical difference was analyzed by Wilcoxon test, P<0.005.