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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Neurosci Methods. 2011 May 11;199(2):199–207. doi: 10.1016/j.jneumeth.2011.05.004

Fig. 2.

Fig. 2

A comparison of responses to the manipulation of microinjection performed in traditional and new described way. Two microinjections of 100 nL of PBS were performed in a group of rats (N=4) using an injector which was inserted immediately before microinjections and removed 60 sec after microinjection (traditional way) or was pre-installed (described in this manuscript) at least 1h before microinjection. The effect of Technique was statistically significant for HR (F(1,3)=12.0, p<0.05) and Locomotor Activity (F(1,3)=19.3, p<0.05). Interaction of Technique and Time was also significant for both HR and Activity. For MBP only interaction between Technique and Time reached the level of significance (F(9,27)=5.7, p<0.001).