Fig 1.
Ano1-immunoreactivity is seen in both the ICC cell body and in the processes (A) Ano1 and Kit-immunoreactivity in the ICC cell body and the processes in colon tissue obtained from a patient with non obstructive colon cancer. The merged image shows Ano1 and Kit-immunoreactivity were coincident. (B) Ano1 and Kit-immunoreactivity in an ICC cell body and its processes in colon tissue obtained from a patient with slow transit constipation. The merged image shows Ano1 and Kit-immunoreactivity were also coincident in slow transit constipation. These images were collected using a 60×, 1.2NA water immersion objective and represent a single section obtained from a confocal stack collected with 0.3 μm z axis steps. The signal obtained with the rabbit antiserum (Ano1) is shown in red and the signal from mouse antiserum (Kit) is shown in green.