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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Cancer Res. 2011 May 25;71(14):4908–4919. doi: 10.1158/0008-5472.CAN-10-3395

Figure 6. SPARC overexpression in medulloblastoma cells inhibits IL-6 signaling and induces expression of neuronal markers.

Figure 6

(A) Medulloblastoma cells were infected with mock, 100MOI Ad-DsRed or the indicated MOI of Ad-DsRed-SP for 36hrs. Cell lysates were assessed for SPARC, IL-6, pSTAT3, and Notch1 by immunoblot analysis using specific antibodies. GAPDH served as a control. Protein band intensities were quantified by using ImageJ software (NIH). Columns, mean of three experiments; bars, SD; *, significant difference from Ad-DsRed (p<0.01). (B) Cells were transfected with a plasmid expressing IL-6 for 16hrs and infected with mock, 100MOI Ad-DsRed or the indicated MOI of Ad-DsRed-SP for an additional 24hrs. Cell lysates were assessed for neuronal markers by using their specific antibodies. Results are representative of three independent experiments. Protein band intensities were quantified by using ImageJ software (NIH). Columns, mean of three experiments; bars, SD; *, significant difference from Ad-DsRed (p<0.01); **, significant difference from Ad-DsRed-SP (p<0.05), (C) Cells were infected with mock, 100MOI control siRNA or SP-siRNA for 24hrs and treated with IL-6 blocking antibody for further 12hrs. Cell lysates were assessed for IL-6, Notch1 and neuronal markers by using their specific antibodies. Results are representative of three independent experiments. Protein band intensities were quantified by using ImageJ software (NIH). Columns, mean of three experiments; bars, SD; Inline graphic, significant difference from control-siRNA (p<0.01); @, significant difference from SP-siRNA (p<0.01)].