A. Fluorescent intensity of the liver in 18 rats injected with 0.04, 0.08 or 0.16 after 24, 48, 72 and 96 h.
B. Fluorescent intensity of all liver tumors (N = 34) in 18 rats injected with 0.04, 0.08 or 0.16 mg ICG after 24, 48, 72 and 96 h.
C. Average tumor-to-liver ratios and standard deviations are plotted for all liver metastases (N = 34) in 18 rats injected with 0.04, 0.08 or 0.16 mg ICG after 24, 48, 72 and 96 h.
D. Estimated marginal means of tumor-to-liver ratio for rats injected with 0.04, 0.08 or 0.16 mg ICG after 24, 48, 72 and 96 h using the repeated measures ANOVA and least square difference (LSD) adjustment for multiple testing. This model showed that the tumor-to-liver ratio was significantly higher in the 72 h time group compared to the 24 h (P < 0.001), 48 h (P = 0.001) and 96 h (P = 0.004) time groups. ICG dose did not significantly influence tumor-to-liver ratios, but a trend was found favoring the 0.08 mg dose group (0.08 vs. 0.04, P = 0.06; 0.08 vs. 0.16, P = 0.09).