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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Eur J Neurosci. 2011 Jun 30;34(2):263–271. doi: 10.1111/j.1460-9568.2011.07741.x

FIG. 1.

FIG. 1

Immunoblot detection of Cx26 and Cx30 in homogenates of the liver and thalamus, and in lysates of C6 glioma cells transiently transfected with Cx30 (C6-Cx30) or stably expressing Cx26 (C6-Cx26). Lanes were loaded with 30 µg of protein from liver or thalamus, and with 15 µg of protein from C6 cells. (A,B) Anti-Cx26 antibodies Ab33-5800 (A) and Ab51-2800 (B) detect Cx26 migrating at 26 kDa in liver (lanes 1, 6), thalamus (lanes 2, 7) and C6-Cx26 cells (lanes 3, 8), which was absent in C6-Cx30 (lanes 4, 9) and in control vector-transfected C6 cells (lanes 5, 10). (C) Anti-Cx30 Ab71-2200 detected Cx30 migrating at 30 kDa in the thalamus (lane 11) and in C6-Cx30 cells (lane 12), but not in C6-Cx26 cells (lane 13) or in control vector-transfected C6 cells (C6-vector, lane 14).