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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Eur J Neurosci. 2011 Jun 30;34(2):263–271. doi: 10.1111/j.1460-9568.2011.07741.x

FIG. 4.

FIG. 4

Immunofluorescence localization of labelling for Cx26 with GFAP, Cx43 and Cx47 in brain. (A,B) Low magnification overlays showing co-distribution of Cx26 (green) with GFAP-positive astrocytes (red) in rat (A) and mouse (B) brain. (C–E) Higher magnification confocal overlay images showing co-localization of punctate labelling for Cx26 (green) along GFAP-positive astrocyte processes (red) in the subthalamic nucleus (C) and reticular thalamic nucleus (D) of rat, and the reticular thalamic nucleus of mouse (E), with red/green overlap seen as yellow (arrows). (F) Laser scanning confocal double immunofluorescence labelling for Cx26 with Cx43 in mouse reticular thalamic nucleus, showing Cx26-positive puncta (F1) co-localized with Cx43-positive puncta (F2), as seen by yellow in overlays (F3). Laser scanning confocal double immunofluorescence labelling for Cx26 with Cx47 in mouse reticular thalamic nucleus, showing Cx26-positive puncta (G1) co-localized with Cx47-positive puncta (G2), as seen by yellow in overlays (G3). Scale bars: A, 100 µm; B, 50 µm; C–E, F,G, 10 µm.