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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Cell Physiol. 2012 Jan;227(1):278–287. doi: 10.1002/jcp.22733

Figure 4. Dynamic metaphase plate re-arrangement in Ski−/− MEFs arrested in metaphase and in Ski+/− cells.

Figure 4

Mitotic Ski−/− and Ski+/−MEFs were analysed as described in Figure 2. (a) Ski−/− cell arrested in metaphase for more than 120 minutes. Chromosomes remain aligned for 90 min, then a radial metaphase plate is evident and after 10 minutes, an apparently correct metaphase alignment is evident again. (b) A Ski+/− cell showing the same metaphase plate re-organization as in (a), but with different timing and outcome. Time, in minutes (min.), indicates time since first chromosome alignment. Arrows point to misaligned chromosomes. Scale bars: 10 μm. Movies showing the complete process are available as Supplementary videos (S3 and S4).