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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Cell Physiol. 2012 Jan;227(1):278–287. doi: 10.1002/jcp.22733

Figure 5. Spindle Checkpoint activation in Ski+/− and Ski−/− MEFs.

Figure 5

DNA content histogram of Ski+/− (a) and Ski−/− (b) MEFs treated with colcemide (0,2 μg/ml) for 0 to 24 hours. (c) Cyclin B1 levels in Ski+/− and Ski−/− MEFs treated as in (a) determined by western blotting. β-Actin was used as loading control. Cyclin B bands were quantified, normalized to β-Actin and expressed as relative to time 0h (untreated cells). A representative experiment of at least three independent experiments is shown.