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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: J Control Release. 2011 Feb 2;153(3):255–261. doi: 10.1016/j.jconrel.2011.01.028

Figure 7.

Figure 7

pVEGF loaded fibrin hydrogels resulted in enhanced angiogenesis in a CAM model. A fibrin hydrogel with polyplexes containing pVEGF was placed on top of the CAM for 3 days. pVEGF was transferred to cells and produced a high concentration of VEGF at the gel area, which resulted in hyperbranced neovessels (arrow). VEGF diffusing out the gel created a decreasing VEGF gradient around the gel and led to radial neovessels around the gel (arrowheads). (A–C) Gross pictures on the gel edge were recorded before the CAM was infused with FITC-dextran for fluorescent imaging (D–F) at the gel area. Induced neovessels were found both around the gel (B–C, arrow heads) and at the gel area (E–F, arrows) with pVEGF, which were not found in the negative control (No DNA). 50 μg pVEGF at N/P = 15 were used for both the 5 and 15 mg/mL gels. The dashed line outlines the edge of the hydrogel and “G” indicates the gel area. Scale bar: D–F: 200μm.