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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jun 15;410(4):846–851. doi: 10.1016/j.bbrc.2011.06.076

Table 3.

qRT-PCR results for catalase-related genes rpoS, katG, and katE. ΔCt is the threshold difference between each gene and the housekeeping gene rrsG. Fold change indicates the gene transcription difference between the cobB/pCA24N_cobB strain vs. the cobB/pCA24N strain in LB medium at 37°C with 20 mM H2O2 for 10 min. Fold changes are relative to the cobB/pCA24N sample.

Strain name rpoS katG katE
ΔCt Fold change ΔCt Fold change ΔCt Fold change
No stress
cobB/pCA24N 12.36 ± 0.07 1.00 8.96 ± 0.07 1.00 11.8 ± 0.1 1.00
cobB/pCA24N_cobB 11.8 ±0.1 1.47 10.90 ± 0.07 −3.84 12.48 ± 0.04 −1.60
1 min H2O2 treatment
cobB/pCA24N 11.0 ± 0.3 1.00 7.7 ± 0.3 1.00 10.1 ± 0.3 1.00
cobB/pCA24N_cobB 13.5 ± 0.1 −5.66 11.87 ± 0.07 −18.00 13.03 ± 0.08 −7.62
10 min H2O2 treatment
cobB/pCA24N 11.7 ± 0.1 1.00 7.4 ± 0.3 1.00 10.9 ± 0.3 1.00
cobB/pCA24N_cobB 13.2 ± 0.2 −2.83 12.05 ± 0.09 −25.11 13.1 ± 0.1 −4.59