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Pre-steady-state kinetics of AIF reduction by NADH. A) Kinetic traces of the absorbance change at 452 (1) and 700 nm (2) observed during reduction of 22 μM AIF by 200 μM NADH. The obtained first-order reduction rate constants were 0.034±0.002 s-1 (1) and 0.0372±0.027 s-1 (2). B) Dependence of the first-order rate constants for AIF reduction on the concentration of NADH in the absence (open circles) and presence of 5.0 mM NAD+ (filled circles).
