Pre-steady-state kinetics of AIF oxidation by 2,6-dimethyl-1,4-bezoquinone. A) Kinetic traces of the absorbance change at 452 (1,3) and 700 nm (2) during oxidation of 14 μM AIF by 100 μM 2,6-dimethyl-1,4-benzoquinone (1,2), or after mixing of reduced AIF with a pure buffer solution (3). The obtained first-order oxidation rate constants were 0.890±0.002 s-1 (1) and 1.013±0.012 s-1 (2). B) Dependence of the observed rate constant for AIF oxidation on the concentration of 2,6-dimethyl-1,4-benzoquinone.