Fig. 1.

Cell viability of soft tissue sarcomas (STS) cell lines treated with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), MG132 and a combination of both agents. (A, B) Cells were incubated for 24 hr in the presence of different concentrations of TRAIL and/or MG132. ^*p<0.05, compared to control or lower dose of each treatment. Cell viability was quantified by MTT assay; each treatment was performed in triplicate. (C) Adding MG132 to TRAIL augmented the apoptosis compared to exposure to a single agent, TRAIL or MG132, in TRAIL-sensitive cells (HTB-93) and TRAIL-resistant cells (HTB-82, HT-1080, and HTB-94). ^*p<0.05, compared to TRAIL 10 ng/mL, MG132 1 µM, and MG132 2 µM. (T10/M1: 10 ng/mL TRAIL+1 µM MG132; T10/M2: 10 ng/mL TRAIL+2 µM MG132).