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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Atherosclerosis. 2011 Apr 9;217(1):106–112. doi: 10.1016/j.atherosclerosis.2011.03.017

Fig. 2.

Fig. 2

Expression of cholesterol transporters during lipid loading and depletion.

A) Cell culture medium was changed on day 16 to 2% LPDS-containing medium and cells were then cultured for a further 24 h before SR-BI analysis by Western blotting on day 17. B,C) BMM on day 5 were incubated for 24 h in 0.5% BSA medium without or with acLDL at indicated concentrations followed by analysis of SR-BI and ABCA1 (B) and ABCG1 in triplicate wells for each condition (C). D) BMM were incubated for 24 h in L-cell conditioned medium (Full), 0.5% BSA medium or 0.5% BSA medium containing the indicated concentrations of acLDL and cellular lipid content then determined. Values shown are the mean and S.D of triplicate determinations. The figure is representative of three independent experiments.