Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Atherosclerosis. 2011 Apr 9;217(1):106–112. doi: 10.1016/j.atherosclerosis.2011.03.017

Fig. 3.

Fig. 3

HDL binding and selective lipid uptake in BMM is independent of SR-BI.

A) Cells at day 5 were incubated with 125I/[3H]CEt labeled HDL2b (10 μg/mL) for 4 h at 37°C. HDL cell association, degradation, CE uptake and selective CE uptake were determined. Values shown were the mean and S.D of triplicate determinations. The figure is representative of three independent experiments. B) Cells were incubated with 125I-labeled HDL2b (10 μg/mL) in the absence or presence of “cold” unlabeled HDL2b (500 μg/mL) for 2 h at 4°C, and HDL cell binding was determined. Values shown were the mean and S.D of triplicate determinations. C) Intact BMM cells at day 5 were biotinylated and SR-BI expression in supernatant (S) (intracellular proteins) and Streptavidin pull-down fractions (P) (surface proteins) determined. As a control, CHO cells over-expressing SR-BI were analyzed in parallel. The figure is representative of two independent experiments.