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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Osteoarthritis Cartilage. 2011 Apr 28;19(8):1058–1065. doi: 10.1016/j.joca.2011.04.009

Figure 5.

Figure 5

Controlled in vivo transgene expression following a single intra-articular injection of AAV2. (A,B,C) Bioluminescent images of a representative rat that received AAV-TRE-Luc in the right knee and AAV-CMV-Luc in the left knee 14 days prior. Images shown were taken (A) before addition of Dox to the drinking water (Day 0), (B) 7 days after Dox was added to the drinking water (Day 7) and (C) 7 days after Dox was removed from the drinking water (Day 14). Luciferase expression was stable in the knee joint injected with AAV-CMV-Luc, but varied with Dox administration in the knee joints injected with AAV-TRE-Luc. (D) Bioluminescence measured in the knee joint 14 days post-AAV-TRE-Luc injection. Bioluminescence is represented as a median fold increase over Day 0, when no Dox was present in the drinking water, and interquartile range. Luciferase expression increased with addition of Dox to the drinking water after 7 days (Day 7: Dox (+)). *p=.015 compared to Day 0: Dox (−). After removal of Dox from the drinking water for 7 days (Day 14: Dox (−)), the luciferase signal decreased. #p=.023 compared to Day 7: Dox (+). (E) Median and interquartile range of bioluminescence measured 13 months post-injection with AAV-TRE-Luc. Luciferase expression increased 7 days after Dox was present in the drinking water (Day 7: Dox (+)). *p=.003 compared to Day 0: Dox (−)). Luciferase expression decreased 7 days after removal of Dox (Day 14: Dox (−)). #p=.016 compared to Day 7: Dox (+). N=4 rats.