Table 3.
T-cell responses of immunized mice from the day before intravaginal challenge with C. trachomatis MoPna
| Antigen | Immunization route (times) |
T-cell proliferative responsea (×103 cpm) to: | |
|---|---|---|---|
| EBb,c | Mediumc | ||
| rMOMP | i.m./s.c. (4X) | 1.9 ± 0.8 | 0.5 ± 0.70 |
| rMOMP | i.vag./col. (2X) + i.m./s.c. (2X) |
3.0 ± 0.8d | 0.2 ± 0.05 |
| rMOMP | i.n./s.l. (2X) + i.m./s.c. (2X) |
4.8 ± 2.3d | 0.2 ± 0.09 |
| MoPn | i.n. (1X) | 3.8 ± 0.8d | 0.1 ± 0.08 |
| Ng-rPorB | i.m./s.c. (4X) | 0.8 ± 0.4 | 0.1 ± 0.08 |
a
The values are means ± 1 SD for the triplicate cultures.
b
The ratio of UV-inactivated C. trachomatis MoPn EB to antigen presenting cells was 10:1.
c
Values are from for a pooled sample of 2 mice run in triplicate.
d
P<0.05 as determined by the Student’s t-test compared to the Ng-rPorB immunized group.