Figure 5.

Effect of globular adiponectin on the production of interleukin (IL)-6 and IL-8 induced by lipopolysaccharide (LPS) in periodontal ligament and gingival fibroblasts. Periodontal ligament fibroblasts (A) and gingival fibroblasts (B) were cultured for 24 hours in the presence of murine myeloma cell-derived globular adiponectin (M-gAd, 15 µg/mL) and then stimulated for 24 hours with LPS (100 ng/mL). The levels of IL-6 and IL-8 in the culture supernatant were assayed by enzyme-linked immunosorbent. ^a)Significantly different from untreated cells (P<0.05).