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. 2011 Apr 11;589(Pt 12):3023–3037. doi: 10.1113/jphysiol.2010.202432

Figure 3. eIF2Bɛ activity is sufficient to increase cap-dependent translation in vitro.

Figure 3

L6 myoblast cells were transfected with a CMV-driven Renilla luciferase reporter and either GFP, or wild-type eIF2Bɛ (2Bɛ), or a mutant lacking the C-terminal catalytic domain (2Bɛ-ΔC). Translation of the luciferase reporter is driven exclusively by a cap-dependent mechanism, and this technique has been shown previously to be reflective of overall protein synthesis rates (Balachandran & Barber, 2004). Twenty-four hours after transfection cells were cultured in 0.5% FBS for an additional 24 h and harvested for the assays indicated. A, RT-PCR showing similar mRNA expression of the luciferase reporter among conditions. B, Renilla luciferase activity was determined and expressed relative to GFP control. Values are means ± SEM, n = 4. *Different from GFP control, P < 0.05.