Table 2.
Spectroscopic properties and yields of the CYP102A1 M11 mutants
| No. | Residue | Reduced-CO difference spectra | P450 yield (nmol/L growth medium) | |
|---|---|---|---|---|
| Maximum (nm) | A 450/A 420 ratio | |||
| Nonpolar side chain | ||||
| 1 | Phe87 | 450 | >100 | 165 |
| 2 | Gly87 | 423, 449 | 78 | 148 |
| 3 | Ala87 | 449 | >100 | 51 |
| 4 | Leu87 | 449 | >100 | 237 |
| 5 | Ile87 | 449 | >100 | 581 |
| 6 | Val87a | 449 | >100 | 666 |
| 7 | Met87 | 423, 448 | 12.1 | 344 |
| 8 | Pro87 | 420 | ND | NQ |
| 9 | Trp87 | 450 | >100 | 27 |
| Uncharged polar side chain | ||||
| 10 | Ser87 | 422 | ND | NQ |
| 11 | Thr87 | 449 | >100 | 203 |
| 12 | Asn87 | 421, 450 | 1.2 | 132 |
| 13 | Gln87 | 448 | >100 | 49 |
| 14 | Tyr87 | 448 | >100 | 585 |
| 15 | Cys87 | 450 | >100 | 41 |
| Charged polar side chain | ||||
| 16 | Lys87 | 448 | >100 | 91 |
| 17 | Arg87 | 449 | >100 | 173 |
| 18 | His87 | 423, 450 | 5.8 | 80 |
| 19 | Asp87 | 420 | ND | NQ |
| 20 | Glu87 | 420 | ND | NQ |
ND no detectable peak at 450 nm, NQ not quantifiable owing to the absence of a peak at 450 nm
aCYP102A1 M11 containing R47L, E64G, F81I, F87V, E143G, L188Q, E267V, and G415S [9]