Table 4.

Specific activities (nmol NADPH/min/nmol P450) of alkoxyresorufin-induced NADPH consumption by CYP102A1 M11 mutants and wild-type CYP102A1

No.	Residue	Side chain									DMSO
		Methyl	Ethyl	n-Propyl	n-Butyl	n-Pentyl	n-Hexyl	n-Heptyl	n-Octyl	Benzyl
Nonpolar side chain
1	Phe87	4,100	4,590	3,640	4,520	4,290	3,120	1,150	370	2,170	<10
2	Gly87	4,380	3,910	3,950	4,440	4,170	2,200	960	300	2,360	105
3	Ala87	1,860	1,800	1,510	1,910	1,620	550	300	130	950	90
4	Leu87	1,180	1,120	960	1,110	540	260	270	190	190	95
5	Ile87	820	930	770	730	360	180	100	110	200	85
6	Val87a	2,930	2,660	2,590	3,180	2,100	900	290	310	720	190
7	Met87	1,030	760	660	920	880	140	110	75	400	25
8	Pro87	–	–	–	–	–	–	–	–	–	–
9	Trp87	2,090	1,790	1,450	1,880	1,980	870	320	130	1,450	45
Uncharged polar side chain
10	Ser87	–	–	–	–	–	–	–	–	–	–
11	Thr87	1,910	1,570	1,530	1,900	1,890	800	470	260	480	<10
12	Asn87	3,260	2,950	2,450	2,920	2,970	1,530	730	250	2,260	<10
13	Gln87	1,860	1,740	1,510	1,770	1,920	700	420	200	1,130	40
14	Tyr87	700	680	620	710	690	400	130	87	370	<10
15	Cys87	3,570	3,490	3,030	3,430	3,470	1,590	560	150	2,370	35
Charged polar side chain
16	Lys87	3,570	3,400	3,080	4,100	3,780	1,580	600	210	2,220	<10
17	Arg87	3,740	3,780	2,950	3,400	2,970	840	240	97	1,330	25
18	His87	6,110	6,080	5,650	5,600	5,600	2,240	1,650	890	5,100	<10
19	Asp87	–	–	–	–	–	–	–	–	–	–
20	Glu87	–	–	–	–	–	–	–	–	–	–
21	Wild type (Phe87)	4,650	3,970	3,290	4,220	4,020	2,260	1,030	730	4,110	45

The values represent averages of three measurements; standard deviations were less than 10%

DMSO dimethyl sulfoxide

^aCYP102A1 M11 containing R47L, E64G, F81I, F87V, E143G, L188Q, E267V, and G415S [22]