Skip to main content
. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: J Mol Biol. 2011 Jul 22;410(4):716–725. doi: 10.1016/j.jmb.2011.04.069

FIGURE 1. Spry2 inhibits PI(4,5)P2 hydrolysis.

FIGURE 1

Panels A–D. COS-12A2 cells were mock-treated with PBS (panel A) or incubated for 24 hr in media containing either DMSO alone (panel B) or 0.5 μM U73122 or U73343 in DMSO (panels C and D). Samples in panels B–D were prepared at 4 °C; samples in panels E–J were prepared at 23 °C. The mock-treated sample in panel A was stained for Gag with anti-capsid (CA) antibody to demonstrate that the cell line expresses Gag constitutively. Cells in panels B-J were stained with anti-PI(4,5)P2 antibody. Panels H–J. COS-12A2 cells were transfected with DNA encoding HA-tagged Spry2. HA-Spry2 (green) and PI(4,5)P2 (red) were visualized by indirect immunofluorescence using FITC- or TRITC-labeled secondary antibodies targeted to primary antibodies against the HA tag on Spry2 or the phospholipid, respectively. The bar measures 10 μm.