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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: J Mol Biol. 2011 Jul 22;410(4):716–725. doi: 10.1016/j.jmb.2011.04.069

FIGURE 4. Co-expression of Gag with WT Spry2 inhibits VLP release; inhibition is relieved by mutation of the PI(4,5)P2 and PLCγ-binding regions.

FIGURE 4

COS-1 cells were transfected with DNA encoding WT HIV-1 Gag alone (lane 1) or with DNA encoding Gag and WT Spry2 (lanes 2 and 3), Spry2-Δ36–66 (lanes 4 and 5) or Spry2-R252D (lanes 6 and 7) at spry2-gag ratios of 1:1 or 2:1. Forty eight hours after transfection, the cells were harvested and media analyzed. Media and cell lysates were examined for VLP production (panel A) and expression of Gag-HA in the cytoplasm (panel B) by Western analysis. Panel C, Quantitative analysis of VLP release efficiency.