Figure 3.

Effects of genistein and daidzein on Gadd45 and COX-2 gene expression in BJ-5ta cells irradiated with 60 mJ/cm² UVB. mRNA levels of Gadd45 and COX-2 were determined by Real Time-PCR in BJ-5ta cells treated for 2 h with various concentrations of genistein (from 2 to 10 μM) (a) or daidzein (from 8 to 30 μM) (b) or with different combinations of genistein and daidzein (c) before UVB irradiation (60 mJ/cm²) and harvested 24 h after UVB. The bars indicate the relative abundance of the mRNA; +1 is the abundance of Gadd45 and COX-2 mRNA in unirradiated and untreated cells. All values represent the mean ± SD of triplicate experiments. *P < 0.05, **P < 0.001, ***P < 0.0001, °°°P < 0.0001 (Ctrl: untreated and unirradiated control cells; without substance: untreated and irradiated (60 mJ/cm²) cells).