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Design of pre-miRNA-based shRNAs and their RNAi activity. (a) The set of four synthetic shRNAs containing a 21 or 24 bp stem, either with or without a 2 bp deletion of the 3′ arm, is represented. The underlined sequences are complementary to the LMNA mRNA sequence. The lower cases are derived from the human pre-miR-155 sequence. (b) The RNAi activity of these shRNAs targeting LMNA was analyzed by immunoblotting. TF is the abbreviation for transfection.
