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. Author manuscript; available in PMC: 2011 Jul 19.
Published in final edited form as: J Invest Dermatol. 2010 Sep 2;131(1):84–98. doi: 10.1038/jid.2010.253

Figure 2. Platelet-derived growth factor-BB (PDGF-BB)-bound fibronectin (FN) recombinant domains that included fibronectin type III repeats: FNIII1, FNIII13–14, or fibronectin variable domain (V).

Figure 2

Real-time interaction of PDGF-BB with FN domains was determined by plasmon surface resonance (BiaCore 2000). FN domains were injected (6.25–200 nm, 30 µl minute−1) across a biosensor chip to which PDGF-BB had been conjugated. Chip cell without PDGF-BB was used as a reference. Sensorgrams are representative of three different experiments. Dissociation constants (KDs) were derived from the ratio of kinetic dissociation constants (kd) divided by kinetic association constants (ka). RU, resonance unit.