Figure 2.

Release of PGE₂ from peripheral blood mononuclear cells treated with PDAC supernatants. Supernatants (SN) from AsPC-1 and MiaPaCa-2 cells, derived 24 hrs after plating of the cells, were treated (SN + cPLA2 Inh.) or not (SN) with Pyrolidin 1 and applied onto cultured PBMCs (1 × 10⁶ cells/well) in a 24 well plate. Two parts of PDAC medium (400 μL) were added onto one part medium overlaying the PBMCs (200 μL) and 48 hrs later samples were collected and analysed for PGE₂ expression. The experiment was repeated in duplicate with three different healthy blood donors. *Standard deviations were calculated and results were considered significant with P values from Student's t-test below 0.05.