Fig. 1. MyD88 is a component of the NF-κB and apoptotic pathways initiated by BLP through hTLR2. (A and B) 293 cells were transiently co-transfected with equivalent amounts (0.25 µg) of expression plasmids encoding gD-TLR2 or gD-TLR2Δ2 and the indicated dominant negatives. An NF-κB-regulated luciferase reporter plasmid was also transfected. At 24 h post-transfection, the cells were incubated in medium alone (white bars) or in medium with 1 µg/ml sBLP (black bars). The induction of NF-κB-dependent transcription (A) was determined. Apoptosis (B) was measured by TUNEL analysis. Data in (B) are reported as the percentage specific apoptosis relative to cells transfected with gD-TLR2Δ2 without sBLP. Results are the average ± SD of two independent samples. (C) Anti-gD western blot of lysates prepared from cells transfected as in (A) and (B).