Fig. 7. TLR2 mediates pro-IL-1β synthesis and caspase 1 activation in THP-1 cells treated with sBLP. THP-1 cells were pre-treated for 6 h with 32 nM PMA in the indicated conditions. Where shown, cells were pre-incubated with 10 µM Ac-YVAD-cmk, 25 µg/ml anti-TLR2 mAb 2392 or isotype control mAb and then treated with sBLP at 4 ng/ml. After 3 h, cell lysates were resolved by SDS–PAGE and blotted for pro-IL-1β. Culture supernatants were collected, resolved by SDS–PAGE and blotted for mature IL-1β. As a control for the molecular weight of mature IL-1β, 1 ng of recombinant mature IL-1β was resolved and blotted in parallel.